Signalling

Part:BBa_K549003:Design

Designed by: Sabine Wagner   Group: iGEM11_LMU-Munich   (2011-09-12)


Nickel(II) dependent promotor with luxAB as reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 751
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1270


Design Notes

There where no special considerations we had to deal with.


Source

The RcnA promotor is amplified from E. coli K12 The luxAB reporter gene is abstracted from the BB_K216008.

References